t cell transact tm (Miltenyi Biotec)

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Miltenyi Biotec t cell transact tm
T Cell Transact Tm, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 553 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t+cell+transact/T+Cell+TransAct+%2C+human/pm42537011-57-30-34
Average 97 stars, based on 553 article reviews

t cell transact tm - by Bioz Stars, 2026-09

97/100 stars

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Activation Assay:

Article Title: H3K18 lactylation drives CD8 + T cell exclusion and anti-PD-1 resistance in hepatocellular carcinoma.
Article Snippet: .. For T cell activation and expansion, cultures were established in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum, 20 IU/mL IL-2 (PeproTech), 1:100 T cell TransAct (Miltenyi Biotec), 50 U/mL penicillin, and 50 μg/mL streptomycin (Gibco). ..

Article Title: A multidimensional platform to support intestinal epithelial and immune cell co-culture
Article Snippet: .. T cell TransAct, human (Miltenyi, 130-111-160) was used to induce activation and activation status was characterized by flow cytometry. ..

Concentration Assay:

Article Title: Selection of human hematopoietic stem cells bearing the intended functional edit by transient AND-gate reporters.
Article Snippet: .. First, 3 days after stimulation with T cell TransAct 60 μl ml−1 (Miltenyi Biotec), cells were edited at the IL2RG locus using the P3 primary cell 4D-Nucleofector X kit and Nucleofector 4D device with RNPs Nature Biotechnology Article https://doi.org/10.1038/s41587-026-03142-z at a final concentration of 2.5 μM. ..

Isolation:

Article Title: Dual-targeting LILRB3/LILRB4 CAR-T cells for the treatment of monocytic acute myeloid leukemia.
Article Snippet: Monocytic acute myeloid leukemia (AML) is an aggressive malignancy associated with poor prognosis.. High expression levels of LILRB3 and LILRB4 are commonly observed in monocytic AML.. In this study, the expression of LILRB3 and LILRB4 was analyzed in monocytic AML patient samples, leukemic stem cell-enriched populations, and AML cell lines, revealing frequent co-expression and suggesting that a dualtargeting approach may provide enhanced therapeutic benefit.

Article Title: B7 homolog 3-targeted CAR-T cells secreting EGFR T-cell engagers for improved control of glioblastoma progression
Article Snippet: Lentiviruses were generated by transfecting 293 T cells with the plasmid encoding the CAR-BsTe constructs, a plasmid encoding a VSV-G envelope (pMD2.G), and an empty backbone plasmid (psPAX2) using polyethyleneimine (PEI) Reagent (#408,727, Sigma-Aldrich) as previously described [ ]. .. PBMCs were isolated from patients or healthy donors by Ficoll, and T cells were enriched (EasySep Human T Cell Enrichment Kit; #19,051, StemCell Technologies) and activated with T‐Cell TransAct (#130–111–160, Miltenyi Biotec). .. Cells were cultured in X‐VIVO 15 medium (#02-060Q, Lonza) with 8% FBS and 20 U/mL IL‐2 (#200–02-50UG, PeproTech) for 3 days.

Gradient Centrifugation:

Transduction:

Flow Cytometry:

Cell Culture:

Article Title: A comparative and exploratory analysis of computational methods for TCR structural prediction and antigen-specific TCR discovery
Article Snippet: .. Human splenocytes were activated with T Cell TransACT (Miltenyi, 130-111-160) and cultured in GT-T551 medium (Takara, WK593S) supplemented with 5% heat-inactivated human AB serum (Gemini, 100-512) and 400 U/ml IL-2 (PeproTech, 200-02) for 4 days. .. For transduction, 9 ml viral supernatant was added to RetroNectin-coated (Takara, T100A) 6-well plates and centrifuged at 2000 × g for 1.5 h at 37°C to allow virus adsorption.

Infection:

Article Title: Genotypic and phenotypic characteristics of CXCR4-using transmitted/founder HIV-1 envelope glycoproteins
Article Snippet: For infection experiments, CD4+ T cells in complete RPMI 1640 medium were activated for 2 days in the presence of phytohemagglutinin (PHA, Thermo Fisher Scientific) (1 μg/mL) and interleukin-2 (IL-2, 300 IU/mL, PeproTech), followed by 3 days in the presence of IL-2 alone. .. For infection experiments of PBMCs, cells suspended in complete RPMI 1640 medium were stimulated with anti-CD3/CD28 antibodies (2.5 μL/mL) for 48 h (T Cell TransAct from Miltenyi Biotec) in the presence of 20 IU/mL IL-2. ..

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